Production Of Transgenic Animals Notes

A transgenic animal is an animal in which one or more genes have been introduced into its nonreproductive cells.

Production of transgenic animals notes. The main principle in the production of transgenic animals is the introduction of a foreign gene or genes into an animal the inserted genes are called transgenes. 43 Creation of the transgenic animal A number of methods are currently in use for the creation of transgenic animals. PowerPoint PPT presentation.

The foreign genes must be transmitted through the germ line so that every cell including germ cells of the animal contains the same modified genetic material. The foreign gene is constructed using recombinant DNA methodology. The transgenic expression is not only used to breed animals which have specific traits but also to breed animals that are lacking specific genes This technique is called as knockout or gene.

Many procedures have been developed to increase the efficiency of this generally inefficient process. Transgenic animals can be created by manipulating embryonic stem cells. Applications of Genetically modified animals.

The cells are then incorporated into an embryo at the blastocyst stage of development. Transgenic mice are developed by injecting DNA into the oocytes or 1-2 celled embryos taken from female mice. Example- Transgenic rats rabbits pigs sheep etc.

A Gene requires certain cellular mechanism to help for the production of protein. After injecting the DNA the embryo is implanted into the uterus of receptive females. A transgenic animal is one that carries a foreign gene that has been deliberately inserted into its genome.

Transgene is incorporated into the ES cell by Microinjection By a retro virus By electroporation Transgenic stem cells are grown in vitro. Animals that have had their DNA manipulated to possess and express a foreign gene are known as transgenic animals. The three main ways of producing transgenic animals are pronuclear manipulation embryonic stem ES cell manipulation and.